2019;10:582957.Marston DA, McElhinney LM, Ellis RJ, Horton DL, Wise EL, Leech SL, et al. Methotrexate (MTX) is one of the most widely used medications to treat rheumatoid arthritis (RA), and recent studies have also suggested the potential benefit of the drug for the treatment of osteoarthritis (OA) of the knee. MTX is commonly administered in oral formulations, but is often associated with systemic adverse reactions. Tatsuya T, Yoshinobu H, Hidetomo K, Naoaki M, Ryoichi S, Tadashi M, Haruhiko S. Novel hyaluronic acid-methotrexate conjugate suppresses joint inflammation in the rat knee: efficacy and safety evaluation in two rat arthritis models. Genes Environ. Mol Biol Evol. Genotoxicity of the compound was evaluated in a rat skin micronucleus (MN) test.
2012;743:42–51.ICH steering committee. Chromatin immunoprecipitation followed by deep sequencing (ChIP-seq) is the most widely used method for characterizing the epigenetic states of chromatin on a genomic scale. After a hypotonic treatment with 0.075 mol/L KCl, the cells were fixed with Carnoy’s solution to prepare the slides. 2016;16(1):301. Predicting DNA duplex stability from the base sequence.
The staining density of nuclei on the histopathology slides (C1 and C2) was less than that on the slides for MN observation (A1, A2, B1, and B2) because the sliced section was generally thinner than the width of a nucleusFukuyama K, Kakimi S, Epstein W. Detection a fibrous component in keratohyalin granules of newborn rat epidermis. 2013;30(4):772–80.Kamau E, Agoti CN, Lewa CS, Oketch J, Owor BE, Otieno GP, Bett A, Cane PA, Nokes DJ. 2010;7(2):111–8.Following the publication of this article [1], it was noted that due to a typesetting error the figure legends were paired incorrectly.Conceptualization, JWO, MVTP, DJN and MC; Data curation, EK; Formal analysis, EK and MC; Funding acquisition, DJN; Methodology, EK, JWO, ZRDL and MC; Project administration, DJN; Resources, DJN; Supervision, CNA; Validation, EK and JWO; Writing – original draft, EK, DJN and MC; Writing – review & editing, EK, JWO, ZRDL, MVTP, CNA, DJN and MC. In the 6-h treatment conditions, the doses were set at 0, 12.5, 25, and 50 μg/mL without S9, or 0, 12.2, 36.7, and 110 μg/mL with S9.
To elucidate the causes of the discrepancy, the MN specimens were re-stained with acridine orange (AO), a fluorescent dye specific to nucleic acid, and the in vivo clastogenicity of the compound in rat skin was re-evaluated. Annu Rev Genomics Hum Genet. 1999;444:159–66.All the present study was funded by Chugai Pharmaceutical Co., Ltd.Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.The datasets generated and analyzed during the current study are available from the corresponding author on reasonable request.Hara T, Nishikawa T, Sui H, Kawakami K, Matsumoto H, Tanaka N. In vivo photochemical skin micronucleus test using a sunlight simulator: detection of 8-methoxypsoralen and benzo[a]pyrene in hairless mice. Influenza Other Respi Viruses. Virology. Genetic diversity between human metapneumovirus subgroups. Mutat Res. Clin Infect Dis. 2016;10(5):386–93.Bénet T, Sylla M, Messaoudi M, Sánchez Picot V, Telles J-N, Diakite A-A, et al. 2015;144(3):516–26.Hammitt LL, Kazungu S, Welch S, Bett A, Onyango CO, Gunson RN, et al. 2015;87(2):275–80.We report a 2-reaction amplicon-based next generation sequencing method to determine the complete genome sequences of five HMPV strains, representing three subgroups (A2, B1 and B2), directly from clinical samples. Influenza Other Respi Viruses. Epidermis (ep); dermis (de); cornified layer (co); a keratinocyte layer (ke) consisting of granular, basal, and squamous layers; nucleus (nu); nucleolus (nl); and keratohyalin granules (keg) are indicated. The contralateral, non-immobilized leg served as an internal control.
J Invest Dermatol.